Calreticulin anticorps (C-Term)

N° du produit ABIN5596697

Détail du produit anti-Calreticulin anticorps

Antigène: Calreticulin (CALR)

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Calreticulin est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with human, mouse, rat, primate, and chicken based on 100% sequence homology. Cross-reactivity with Calreticulin precursor from other sources has not been determined.

Purification: Anti-Calreticulin precursor was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogène:

Immunogen: Calreticulin precursor affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human calreticulin precursor protein.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Calreticulin precursor antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~48 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.2 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.42 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Calreticulin

Antigène: Calreticulin (CALR)

Autre désignation: Calreticulin precursor (CALR Produits)

Synonymes: anticorps CRT, anticorps RO, anticorps SSA, anticorps cC1qR, anticorps crc, anticorps crt, anticorps Calregulin, anticorps CG9429, anticorps Clr, anticorps Dmel\\CG9429, anticorps anon-EST:Liang-2.11, anticorps anon-EST:Liang-2.17, anticorps anon-EST:fe3D7, anticorps clone 2.11, anticorps clone 2.17, anticorps eth, anticorps potp, anticorps cc1qr, anticorps ssa, anticorps NV19007, anticorps CRP55, anticorps ERp60, anticorps HACBP, anticorps CHOT1, anticorps CHT1, anticorps CT1, anticorps AA589632, anticorps CRTR, anticorps Creat, anticorps CCDS1, anticorps calrl2, anticorps fc66d10, anticorps wu:fc66d10, anticorps wu:fj50c03, anticorps zgc:66153, anticorps calreticulin, anticorps calreticulin L homeolog, anticorps Calreticulin, anticorps putative calreticulin, anticorps solute carrier family 6 member 8, anticorps solute carrier family 6 (neurotransmitter transporter, creatine), member 8, anticorps CALR, anticorps calr.L, anticorps Crt, anticorps crt, anticorps CRT, anticorps Calr, anticorps calr, anticorps LMJF_31_2600, anticorps Crc, anticorps Slc6a8, anticorps SLC6A8, anticorps LOC8269812

Sujet:

Synonyms: CRP55, Calregulin, Endoplasmic reticulum resident protein 60, Calreticulin precursor, CRTC, gpr60

Background: Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone.

Gene Name: CALR

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response