APOB anticorps

N° du produit ABIN5596981

Détail du produit anti-APOB anticorps

Antigène: APOB (Apolipoprotein B (APOB))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp APOB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Purification: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Immunogène:

Immunogen: apoLipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50 - 1:500

Application Note: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:200 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-APOB (ABIN5596981)

Morishita, Zhao, Tamura, Nishimura, Kanda, Sakamaki, Okazaki, Li, Mizushima: "A critical role of VMP1 in lipoprotein secretion." dans: eLife, Vol. 8, (2020) (PubMed).

Sołtysik, Ohsaki, Tatematsu, Cheng, Fujimoto: "Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis." dans: Nature communications, Vol. 10, Issue 1, pp. 473, (2019) (PubMed).

Lu, Yuan, Wang, Zhang, Yuan, Wu, Zhou, He, Lu, Chen, Fan, Liang, Cheng: "Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7." dans: EBioMedicine, Vol. 36, pp. 29-38, (2019) (PubMed).

Nauli, Sun, Whittimore, Atyia, Krishnaswamy, Nauli: "Chylomicrons produced by Caco-2 cells contained ApoB-48 with diameter of 80-200 nm." dans: Physiological reports, Vol. 2, Issue 6, (2014) (PubMed).

Boyer, Dumans, Beaumont, Etienne, Roingeard, Meunier: "The association of hepatitis C virus glycoproteins with apolipoproteins E and B early in assembly is conserved in lipoviral particles." dans: The Journal of biological chemistry, Vol. 289, Issue 27, pp. 18904-13, (2014) (PubMed).

Détails sur APOB

Antigène: APOB (Apolipoprotein B (APOB))

Autre désignation: APOLIPOPROTEIN B (APOB Produits)

Synonymes: anticorps FLDB, anticorps LDLCQ4, anticorps Ac1-060, anticorps Apo B-100, anticorps ApoB-100, anticorps ApoB-48, anticorps AI315052, anticorps apob-100, anticorps apob-48, anticorps APOB-100, anticorps ApoB(100), anticorps APOB, anticorps apolipoprotein B, anticorps APOB, anticorps Apob

Sujet:

Synonyms: APOB protein antibody, Apolipoprotein B 100 antibody, Apolipoprotein B 48 antibody, Apolipoprotein B antibody, FLDB antibody

Background: Anti Apolipoprotein B antibody recognizes the gene product of APOB that is the primary apolipoprotein of low-density lipoproteins, which is responsible for carrying cholesterol to tissues. While it is unclear exactly what functional role APOB plays in LDL, it is the primary apolipoprotein component and is absolutely required for its formation. What is clear is that the APOB on the LDL particle acts as a ligand for LDL receptors in various cells throughout the body. Through a mechanism that is not fully understood, high levels of APOB can lead to plaques that cause vascular disease (atherosclerosis), leading to heart disease. There is considerable evidence that levels of APOB are a better indicator of heart disease and cardiovascular risk than total cholesterol or LDL. However, primarily for historic reasons, cholesterol, and more specifically, LDL-cholesterol, remains the primary lipid test for the risk factor of atherosclerosis.

Gene Name: APOB

ID gène: 338

UniProt: Q7Z7Q0

Pathways: Lipid Metabolism