Clusterin anticorps

N° du produit ABIN5596991

Détail du produit anti-Clusterin anticorps

Antigène: Clusterin (CLU)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Clusterin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Purification: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Immunogène:

Immunogen: apoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:5,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-Clusterin (ABIN5596991)

Hayashi, Campenot, Vance, Vance: "Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin." dans: The Journal of biological chemistry, Vol. 284, Issue 43, pp. 29605-13, (2009) (PubMed).

Détails sur Clusterin

Antigène: Clusterin (CLU)

Autre désignation: Apolipoprotein J (CLU Produits)

Synonymes: anticorps LOC100136120, anticorps clu, anticorps MGC53055, anticorps zgc:73352, anticorps xclu, anticorps MGC89163, anticorps CLU, anticorps APO-J, anticorps APOJ, anticorps CLI, anticorps CLU1, anticorps CLU2, anticorps KUB1, anticorps NA1/NA2, anticorps SGP-2, anticorps SGP2, anticorps SP-40, anticorps TRPM-2, anticorps TRPM2, anticorps DAG, anticorps RATTRPM2B, anticorps SP40, anticorps TRPM2B, anticorps Trpm2, anticorps Trpmb, anticorps AI893575, anticorps ApoJ, anticorps Cli, anticorps D14Ucla3, anticorps Sgp-2, anticorps Sgp2, anticorps Sugp-2, anticorps GP80, anticorps gpIII, anticorps 40, anticorps clusterin-1, anticorps clusterin L homeolog, anticorps clusterin, anticorps Clusterin, anticorps LOC100136120, anticorps clu.L, anticorps clu, anticorps CLU, anticorps clus, anticorps Clu, anticorps LOC101113728

Sujet:

Synonyms: Clusterin, Complement-associated protein SP-40,40, Complement cytolysis inhibitor, CLI, NA1/NA2, Apolipoprotein J, Apo-J, Testosterone-repressed prostate message 2, TRPM-2, Ku70-binding protein 1, Aging-associated gene 4 protein

Background: This antibody is suitable for cardiovascular research. Cleaved into the following 2 chains: 1- Recommended name: Clusterin beta chain Alternative name(s): ApoJalpha Complement cytolysis inhibitor a chain 2- Recommended name: Clusterin alpha chain Alternative name(s): ApoJbeta Complement cytolysis inhibitor b chain

Gene Name: CLU, APOJ, CLI, KUB1

ID gène: 1191

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling