Separase anticorps (AA 586-701)

N° du produit ABIN564183

Cité dans 7 publications. Cet anticorps anti-Separase Monoclonal Souris (Clone 6H6) (ABIN564183) détecte spécifiquement Separase dans ELISA, WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Separase anticorps (AA 586-701) (ABIN564183)

Antigène: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Separase est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 6H6

Détail du produit anti-Separase anticorps

Épitope: AA 586-701

Fonction: Mouse monoclonal antibody raised against a partial recombinant ESPL1.

Séquence: REELQAYKAV RADTGQERFN IICDLLELSP EETPAGAWAR ATHLVELAQV LCYHDFTQQT NCSALDAIRE ALQLLDSVRP EAQARDQLLD DKAQALLWLY ICTLEAKIQE GIERDR

Réactivité croisée: Humain

Attributs du produit: Antibody Reactive Against Recombinant Protein.

Immunogène: ESPL1 (NP_036423, 586 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: In 1x PBS, pH 7.4

Conseil sur la manipulation: Aliquot to avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Références pour anticorps anti-Separase (ABIN564183)

Mukherjee, Ge, Zhang, Edwards, Sumazin, Sharan, Rao, Medina, Pati: "MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ER?)-positive mammary adenocarcinomas." dans: Oncogene, Vol. 33, Issue 48, pp. 5511-22, (2014) (PubMed).

Mukherjee, Byrd, Brawley, Bielamowicz, Li, Merchant, Maitra, Sumazin, Fuller, Kew, Sun, Powell, Ahmed, Zhang, Pati: "Overexpression and constitutive nuclear localization of cohesin protease Separase protein correlates with high incidence of relapse and reduced overall survival in glioblastoma multiforme." dans: Journal of neuro-oncology, Vol. 119, Issue 1, pp. 27-35, (2014) (PubMed).

Zhang, Scorsone, Ge, Kaffes, Dobrolecki, Mukherjee, Lewis, Berg, Stephan, Pati: "Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy." dans: Journal of biomolecular screening, Vol. 19, Issue 6, pp. 878-889, (2014) (PubMed).

Mukherjee, Ge, Zhang, Huang, Nakamura, Minor, Fofanov, Rao, Herron, Pati: "Separase loss of function cooperates with the loss of p53 in the initiation and progression of T- and B-cell lymphoma, leukemia and aneuploidy in mice." dans: PLoS ONE, Vol. 6, Issue 7, pp. e22167, (2011) (PubMed).

Panigrahi, Zhang, Mao, Pati: "Calpain-1 cleaves Rad21 to promote sister chromatid separation." dans: Molecular and cellular biology, Vol. 31, Issue 21, pp. 4335-47, (2011) (PubMed).

Meyer, Fofanov, Panigrahi, Merchant, Zhang, Pati: "Overexpression and mislocalization of the chromosomal segregation protein separase in multiple human cancers." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 8, pp. 2703-10, (2009) (PubMed).

Zhang, Ge, Meyer, Sethi, Basu, Pradhan, Zhao, Li, Cai, El-Naggar, Baladandayuthapani, Kittrell, Rao, Medina, Pati: "Overexpression of Separase induces aneuploidy and mammary tumorigenesis." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 35, pp. 13033-8, (2008) (PubMed).

Détails sur Separase

Antigène: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Autre désignation: ESPL1

Sujet: Full Gene Name: extra spindle pole bodies homolog 1 (S. cerevisiae)
Synonyms: ESP1,FLJ46492,KIAA0165,SEPARASE,SEPARIN

ID gène: 9700

NCBI Accession: NM_012291