Separase anticorps

N° du produit ABIN7216974

Cet anticorps anti-Separase Polyclonal Lapin (ABIN7216974) détecte spécifiquement Separase dans ELISA, IHC, WB et IF. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Separase anticorps (ABIN7216974)

Antigène: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Separase est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-Separase anticorps

Fonction: Rabbit Anti-Separase Polyclonal Antibody

Specificité: Separase Polyclonal Antibody detects endogenous levels of Separase protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur Separase

Antigène: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Autre désignation: Separase

Sujet: ESPL1, ESP1, KIAA0165, Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, SeparaseStable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21, MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.

Poids moléculaire: 230kD

ID gène: 9700

UniProt: Q14674