LDHB anticorps

N° du produit ABIN568274

L’anticorps Lapin Polyclonal anti-LDHB a été validé pour WB, IP, IF, EIA, RIA, DB et ID. Il convient pour détecter LDHB dans des échantillons de Boeuf (Vache).

Aperçu rapide pour LDHB anticorps (ABIN568274)

Antigène: LDHB (Lactate Dehydrogenase B (LDHB))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LDHB est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB), Immunodiffusion (ID)

Détail du produit anti-LDHB anticorps

Specificité: L-Lactic Dehydrogenase from Bovine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogène: L-Lactic Dehydrogenase is isolated and purified from Bovine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 1.0 mL sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Détails sur LDHB

Antigène: LDHB (Lactate Dehydrogenase B (LDHB))

Autre désignation: LDHB

Sujet: Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: EC=1.1.1.27, L-lactate dehydrogenase B chain, LDH heart subunit, LDH-B, LDH-H, LDHB, Renal carcinoma antigen NY-REN-46

ID gène: 281275

NCBI Accession: NP_776525

UniProt: Q5E9B1

Pathways: L'effet Warburg