C3 anticorps (FITC)

N° du produit ABIN568553

L’anticorps Chèvre Polyclonal anti-C3 a été validé pour WB, IP, EIA, IF, ID et DB. Il convient pour détecter C3 dans des échantillons de Cobaye.

Aperçu rapide pour C3 anticorps (FITC) (ABIN568553)

Antigène: C3 (Complement Component 3 (C3))

Reactivité: Cobaye

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp C3 est conjugé à/à la FITC

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunodiffusion (ID), Dot Blot (DB)

Détail du produit anti-C3 anticorps (FITC)

Specificité: In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of guinea pig serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of guinea pig plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Attributs du produit: Absorption / Emission: 492 nm / 515 nm.
Molar Ratio: Fluorochrome/IgG ~1.6

Purification: Hyperimmune antisera with strong precipitating activity are selected for Fractionation and purification of the IgG fraction containing the bulk of the defined antibody specificity.

Immunogène: C3c is isolated and purified from pooled normal guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore by adding 1 mL of sterile distilled water

Concentration: 10,0 mg/mL

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.

Détails sur C3

Antigène: C3 (Complement Component 3 (C3))

Autre désignation: Complement C3

Sujet: C3 is the most abundant complement protein in guinea pig serum. Its biological function strongly resembles that of C3 in man an other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response. The antiserum is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Consequently the antiserum reacts with both native and activated C3. It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain.Synonyms: CPAMD1, Complement component 3

ID gène: 24232

NCBI Accession: NP_058690

UniProt: P01026

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling