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C5 anticorps (FITC)

L’anticorps Chèvre Polyclonal anti-C5 a été validé pour IHC (fro), IP, EIA, ID et IF. Il convient pour détecter C5 dans des échantillons de Humain.
N° du produit ABIN568577

Aperçu rapide pour C5 anticorps (FITC) (ABIN568577)

Antigène

Voir toutes C5 Anticorps
C5 (Complement Component 5 (C5))

Reactivité

  • 124
  • 33
  • 23
  • 16
  • 2
  • 2
  • 2
Humain

Hôte

  • 114
  • 22
  • 3
  • 3
Chèvre

Clonalité

  • 121
  • 21
Polyclonal

Conjugué

  • 57
  • 13
  • 11
  • 8
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp C5 est conjugé à/à la FITC

Application

  • 77
  • 50
  • 44
  • 39
  • 35
  • 18
  • 13
  • 11
  • 10
  • 9
  • 6
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF)
  • Specificité

    The reactivity of the antiserum is restricted to C5. In immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C5 protein. No reaction is obtained with any other plasma protein component or serum. The antiserum does not cross react with any other component of human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

    Attributs du produit

    Absorption / Emission: 492 nm / 515 nm
    Molar Ratio: Fluorescein/IgG ~1.4

    Purification

    The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.

    Immunogène

    C5 protein is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization

    Isotype

    IgG
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    Restore by adding 1 mL of sterile distilled water.

    Concentration

    10 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives.

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Avoid Repeated thawing and freezing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    C5 (Complement Component 5 (C5))

    Autre désignation

    Complement C5

    Sujet

    C5 component of human complement is a plasma protein consisting of two polypeptide chains, alpha, beta, bound by disulphide bonds. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack complex.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5

    ID gène

    727

    NCBI Accession

    NP_001726

    UniProt

    P01031

    Pathways

    Système du Complément, Carbohydrate Homeostasis
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