IGF1 anticorps (Biotin)

N° du produit ABIN5687628

Cet anticorps Chèvre Polyclonal détecte spécifiquement IGF1 dans WB et ELISA. Il présente une réactivité envers Souris.

Aperçu rapide pour IGF1 anticorps (Biotin) (ABIN5687628)

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IGF1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-IGF1 anticorps (Biotin)

Purification: Anti-mIGF-I specific antibody was purified by affinity chromatography and then biotinylated.

Immunogène: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIGF-I (murine IGF-I).

Information d'application

Indications d'application: ELISA:

To detect mIGF-I by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIGF-I.

Sandwich

To detect mIGF-I by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IGF-I (XP-5159) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIGF-I.

Western Blot:

To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur IGF1

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: IGF-I

ID gène: 16000

UniProt: P05017

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation