Leptin anticorps (Biotin)

N° du produit ABIN5687650

L’anticorps Chèvre Polyclonal anti-Leptin a été validé pour WB et ELISA. Il convient pour détecter Leptin dans des échantillons de Rat.

Aperçu rapide pour Leptin anticorps (Biotin) (ABIN5687650)

Antigène: Leptin (LEP)

Reactivité: Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Leptin est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-Leptin anticorps (Biotin)

Purification: Anti-rat Leptin specific antibody was purified by affinity chromatography and then biotinylated.

Immunogène: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rat Leptin (rat Leptin).

Information d'application

Indications d'application: ELISA:

To detect rat Leptin by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat Leptin.

To detect Rat Leptin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat Leptin (XP-5213) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin.

Western Blot:

To detect rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: Leptin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur Leptin

Antigène: Leptin (LEP)

Autre désignation: Leptin

ID gène: 25608

UniProt: P50596

Pathways: Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process