NGFB anticorps

N° du produit ABIN5688734

Cet anticorps anti-NGFB est un anticorps Souris Monoclonal détectant NGFB dans WB, ELISA et Neut. Adapté pour Humain.

Aperçu rapide pour NGFB anticorps (ABIN5688734)

Antigène: NGFB (Nerve Growth Factor beta (NGFB))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NGFB est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: M85-2B

Détail du produit anti-NGFB anticorps

Purification: This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.

Immunogène: beta-NGF antibody is produced in mice using highly pure (>98%) recombinant human beta-NGF as the immunizing antigen.

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 200 pg/mL of recombinant human β,-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:

To detect hβ,-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ,-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: 4 °C,-20 °C

Stockage commentaire: beta-NGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Détails sur NGFB

Antigène: NGFB (Nerve Growth Factor beta (NGFB))

Autre désignation: bNGF

ID gène: 4803

UniProt: P01138

Pathways: Signalisation NF-kappaB, Signalisation RTK, Regulation of Cell Size