CCL5 anticorps

N° du produit ABIN5689171

L’anticorps Lapin Polyclonal anti-CCL5 a été validé pour WB, ELISA et Neut. Il convient pour détecter CCL5 dans des échantillons de Souris.

Aperçu rapide pour CCL5 anticorps (ABIN5689171)

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL5 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-CCL5 anticorps

Purification: Anti-mRANTES specific antibody was purified by affinity chromatography employing immobilized mRANTES matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRANTES (murine RANTES).

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mRANTES (100.00 ng/mL), a concentration of 6.0 - 8.0 μ,g/mL of this antibody is required.

ELISA:

To detect mRANTES by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mRANTES.

Sandwich:

To detect mRANTES by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine RANTES (XP-5259Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRANTES.

Western Blot:
To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: RANTES antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur CCL5

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Autre désignation: RANTES

Sujet: RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

ID gène: 20304

UniProt: P30882

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration