PRKAA1 anticorps (pSer487)

N° du produit ABIN5689680

Cet anticorps Lapin Polyclonal détecte spécifiquement PRKAA1 dans WB, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PRKAA1 anticorps (pSer487) (ABIN5689680)

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKAA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-PRKAA1 anticorps

Épitope: pSer487

Specificité: This antibody detects endogenous level of AMPKα,1 only when phosphorylated at serine 487.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogène: AMPKa1 (Phospho-Ser487) antibody was raised in was raised against a peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKα,1.

Information d'application

Indications d'application: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store antibody at -20°C for up to one year.

Détails sur PRKAA1

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Autre désignation: AMPKa1

Sujet: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Poids moléculaire: 63 kDa

ID gène: 5562

NCBI Accession: NP_006242

UniProt: Q13131, P54646

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg