KIT Ligand anticorps

N° du produit ABIN5690440

L’anticorps Lapin Polyclonal anti-KIT Ligand a été validé pour WB, ELISA et Neut. Il convient pour détecter KIT Ligand dans des échantillons de Rat.

Aperçu rapide pour KIT Ligand anticorps (ABIN5690440)

Antigène: KIT Ligand (KITLG)

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KIT Ligand est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-KIT Ligand anticorps

Immunogène: Produced from sera of rabbits immunized with highly pure recombinant Rat IP-10. Rat IP-10 specific antibody was purified by affinity chromatography employing an immobilized Rat IP-10 matrix.

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of rat SCF (50.0 ng/mL), a concentration of 1.2-1.8 μ,g/mL of this antibody is required. ELISA:
Indirect:
To detect rat SCF by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF.

Sandwich
To detect rat SCF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat SCF (38-258) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF.

Western Blot:
To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur KIT Ligand

Antigène: KIT Ligand (KITLG)

Autre désignation: SCF

ID gène: 60427

UniProt: P21581

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway