IDE anticorps (AA 485-756)

N° du produit ABIN5693119

Détail du produit anti-IDE anticorps

Antigène: IDE (Insulin-Degrading Enzyme (IDE))

Épitope: AA 485-756

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IDE est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Marque: Picoband™

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for IDE detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human,Mouse,Rat.

Immunogène: E. coli-derived human IDE recombinant protein (Position: F485-K756).

Information d'application

Indications d'application:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry(Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry, 1-3 μg/1x10⁶ cells
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Détails sur IDE

Antigène: IDE (Insulin-Degrading Enzyme (IDE))

Autre désignation: IDE (IDE Produits)

Synonymes: anticorps zgc:162603, anticorps IDE, anticorps 1300012G03Rik, anticorps 4833415K22Rik, anticorps AA675336, anticorps AI507533, anticorps INSDEGM, anticorps INSULYSIN, anticorps insulin degrading enzyme, anticorps insulin-degrading enzyme, anticorps metallopeptidase, anticorps IDE, anticorps ide, anticorps LOC591315, anticorps CC1G_01115, anticorps Bm1_26615, anticorps PTRG_10875, anticorps SJAG_01430, anticorps EBI_21700, anticorps VDBG_06702, anticorps LOAG_08082, anticorps PGTG_04982, anticorps Tsp_00424, anticorps Ide

Sujet:

Synonyms: Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE

Background: Insulin-degrading enzyme, also known as IDE, is an enzyme. This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt: P14735

Pathways: SARS-CoV-2 Protein Interactome