RPS6KB1 anticorps (C-Term)

N° du produit ABIN5706812

N° du produit (Fournisseur): 600-401-942

L’anticorps Lapin Polyclonal anti-RPS6KB1 a été validé pour WB, ELISA et IP. Il convient pour détecter RPS6KB1 dans des échantillons de Souris.

Aperçu rapide pour RPS6KB1 anticorps (C-Term) (ABIN5706812)

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPS6KB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-RPS6KB1 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-942

Fournisseur: Rockland

Fonction: p70 S6 Kinase Antibody

Réactivité croisée (Details): This affinity-purified antibody is directed against the human p70S6K protein.

Attributs du produit: Synonyms: p70S6Kb antibody, Ribosomal protein S6 kinase 70 kDa, polypeptide 2 antibody, Ribosomal protein S6 kinase beta 2 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p70S6K protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 70 kDa in size corresponding to p70S6K by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

Immunoprecipitation Dilution: 5 μg/mg protein sample

ELISA Dilution: 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur RPS6KB1

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Autre désignation: RPS6KB1

Sujet: Background: The protein p70 S6 kinase is critical for cell cycle progression and cell survival. In response to mitogen stimulation, p70 S6 kinase activation up-regulates ribosomal biosynthesis and enhances the translational capacity. p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome. This kinase was first characterized as an insulin/mitogen-activated protein kinase, whose major known substrate is the 40S ribosomal subunit protein S6. p70S6K is activated by diverse stimuli through multi-site phosphorylation at positions such as Thr-252 and Ser-434. In Alzheimer's Disease, p70S6K activation is associated with PHF-tau (hyperphosphorylated tau) accumulation. In non-neuronal cells, p70S6K has been shown to regulate actin polymerization.

ID gène: 6198, 54041234

UniProt: P23443

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, L'effet Warburg