Cyclin D3 anticorps (C-Term)

N° du produit ABIN5706819

N° du produit (Fournisseur): 600-401-gn2

Cet anticorps anti-Cyclin D3 est un anticorps Lapin Polyclonal détectant Cyclin D3 dans WB, ELISA, IHC, FACS et IP. Adapté pour Humain et Souris.

Aperçu rapide pour Cyclin D3 anticorps (C-Term) (ABIN5706819)

Antigène: Cyclin D3 (CCND3)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin D3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Détail du produit anti-Cyclin D3 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-gn2

Fournisseur: Rockland

Fonction: Cyclin D3 Antibody

Réactivité croisée (Details): Cyclin D3 affinity-purified antibody is directed against Cyclin D3 protein.

Attributs du produit: Synonyms: rabbit anti-Cyclin D3 antibody, G1/S-specific cyclin-D3, Ccnd3, Cyl-3

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Flow Cytometry Dilution: 4 μg/mL

Immunohistochemistry Dilution: 4 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, flow cytometry, and by immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 32.4 kDa in size corresponding to Cyclin D3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Cyclin D3.

Western Blot Dilution: 1.0 μg/mL

Immunoprecipitation Dilution: 5 μg/mL

ELISA Dilution: 1:1000 - 1:5000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Cyclin D3

Antigène: Cyclin D3 (CCND3)

Autre désignation: Ccnd3

Sujet: Background: Anti-Cyclin D3 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Cyclin D3 belongs to a highly conserved cyclin family, whose members are the ultimate recipients of oncogenic signals. Cyclin D3 is a key component of the cell cycle progression machinery and induces progression through the G1 phase of the cell cycle. Cyclin D3 is expressed in nearly all proliferating cells, and shows the most broad expression pattern of all three D-type (D1-D3) cyclins. Cyclin D3 is encoded from the 6p21 chromosome region and the protein is predominantly localized in the nucleus. Once induced, cyclin D3 binds and activates its associated cyclin-dependent kinases CDK4 and CDK6. Amplification of the cyclin D3 gene and overexpression of cyclin D3 protein is seen in several human cancers. A large number of human malignancies contain lesions in pathways impacting on cyclin D3. Abnormal expression of Cyclin D3 is believed to be a driving force in several human cancers. A possible role for cyclin D3 in the malignancies of the lymphoid system is suggested by the observations that cyclin D3 gene is rearranged in several neoplastic diseases such as diffuse large B cell lymphomas or multiple myelomas. Anti-Cyclin D3 is ideal for researchers interested in Cancer Research and Immunology research.

ID gène: 12445

UniProt: P30282

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process