E2F8 anticorps (Internal Region)

N° du produit ABIN570707

L’anticorps Chèvre Polyclonal anti-E2F8 a été validé pour ELISA, FACS et IF. Il convient pour détecter E2F8 dans des échantillons de Humain.

Aperçu rapide pour E2F8 anticorps (Internal Region) (ABIN570707)

Antigène: E2F8 (E2F Transcription Factor 8 (E2F8))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp E2F8 est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-E2F8 anticorps

Épitope: Internal Region

Fonction: E2F8

Séquence: KSIESDRRKI NSAPS

Réactivité croisée: Boeuf (Vache), Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-KSIESDRRKINSAPS , from the internal region of the protein sequence according to NP_078956.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Preliminary testing showed a band at approx 80 kDa in Human K562 and Mouse Neuro2a cell lysate after 0.3 μg/mL antibody staining. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur E2F8

Antigène: E2F8 (E2F Transcription Factor 8 (E2F8))

Autre désignation: E2F8

Sujet: E2F8, E2F transcription factor 8, FLJ23311, E2F family member 8

ID gène: 79733, 108961

NCBI Accession: NP_078956