L’anticorps Lapin Monoclonal anti-Retinoblastoma Protein (Rb) a été validé pour IHC (p). Il convient pour détecter Retinoblastoma Protein (Rb) dans des échantillons de Humain et Souris.
Rb Protein
Reactivité: Humain, Boeuf (Vache), Chien, Porc, Roussette (Chauve-souris), Cobaye
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Due to variation in protocol and secondary antibody used, the recombinant RB1 antibody may need to be titered for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistology (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Restrictions
For Research Use only
Buffer
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
The recombinant RB1 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Antigène
Retinoblastoma Protein (Rb) (Rb Protein)
(Retinoblastoma Protein (Rb Protein))
Autre désignation
Retinoblastoma / Rb
Sujet
Recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.