Retinoblastoma Protein (Rb) anticorps

N° du produit ABIN5708009

Cet anticorps Lapin Monoclonal détecte spécifiquement Retinoblastoma Protein (Rb) dans IHC (p). Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour Retinoblastoma Protein (Rb) anticorps (ABIN5708009)

Antigène: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Retinoblastoma Protein (Rb) est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1-2313R

Détail du produit anti-Retinoblastoma Protein (Rb) anticorps

Purification: Purified

Pureté: Protein A affinity chromatography

Immunogène: A human recombinant partial protein was used as immunogen for this recombinant RB1 antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: Due to variation in protocol and secondary antibody used, the recombinant RB1 antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistology (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: The recombinant RB1 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Détails sur Retinoblastoma Protein (Rb)

Antigène: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Autre désignation: Retinoblastoma / Rb

Sujet: Recognizes a 105  kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Pathways: Mitotic G1-G1/S Phases