Desmoplakin anticorps (Internal Region)

N° du produit ABIN570871

Cet anticorps Chèvre Polyclonal détecte spécifiquement Desmoplakin dans IHC et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour Desmoplakin anticorps (Internal Region) (ABIN570871)

Antigène: Desmoplakin (DSP)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Desmoplakin est non-conjugé

Application: Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-Desmoplakin anticorps

Épitope: Internal Region

Fonction: Desmoplakin (aa 1672 -1684)

Séquence: QESVKQAHLR NEH

Specificité: This antibody is expected to recognize reported isoform I (NP_004406.2) only.

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: C-QESVKQAHLRNEH

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Desmoplakin

Antigène: Desmoplakin (DSP)

Autre désignation: DSP

Sujet: DSP, desmoplakin, DPI, 250 kDa paraneoplastic pemphigus antigen, desmoplakin I

ID gène: 1832, 109620, 306871

NCBI Accession: NP_004406

Pathways: Cell-Cell Junction Organization