MCAM anticorps (CF®594)

N° du produit ABIN6151311

Cet anticorps Souris Monoclonal détecte spécifiquement MCAM dans FACS, IHC et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour MCAM anticorps (CF®594) (ABIN6151311)

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCAM est conjugé à/à la CF®594

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: C146-634

Détail du produit anti-MCAM anticorps (CF®594)

Fonction: Mouse Monoclonal anti-CD146 / MCAM / MUC18 / Mucin 18 (C146/634), CF594 Conjugate

Attributs du produit: The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human CD146 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur MCAM

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Autre désignation: CD146 / MCAM / MUC18 / Mucin 18

Poids moléculaire: 130 kDa

ID gène: 4162, 599039

UniProt: P43121