CD59 anticorps (CF®647)

N° du produit ABIN6151675

L’anticorps Souris Monoclonal anti-CD59 a été validé pour FACS et IF. Il convient pour détecter CD59 dans des échantillons de Humain.

Aperçu rapide pour CD59 anticorps (CF®647) (ABIN6151675)

Antigène: CD59

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD59 est conjugé à/à la CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: BRA-10G

Détail du produit anti-CD59 anticorps (CF®647)

Fonction: Mouse Monoclonal anti-CD59 / Protectin Heavy Chain (BRA-10G), CF647 Conjugate

Attributs du produit: This antibody reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human K562 tumor cells

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur CD59

Antigène: CD59

Autre désignation: CD59 (heavy chain of Protectin)

Poids moléculaire: 20 kDa

ID gène: 966, 278573

UniProt: P13987

Pathways: Système du Complément