Recombinant Melanoma gp100 anticorps (CF®647)

N° du produit ABIN6154753

L’anticorps Lapin Monoclonal anti-Melanoma gp100 a été validé pour IHC, IF et FACS. Il convient pour détecter Melanoma gp100 dans des échantillons de Humain.

Aperçu rapide pour Recombinant Melanoma gp100 anticorps (CF®647) (ABIN6154753)

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Melanoma gp100 est conjugé à/à la CF®647

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PMEL-1825R

Détail du produit anti-Melanoma gp100 anticorps (CF®647)

Fonction: Recombinant Rabbit Monoclonal anti-gp100 / Melanosome / PMEL17 / SILV (PMEL/1825R), CF647 Conjugate

Attributs du produit: Cytotoxic T lymphocytes (CTLs) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human SILV protein

Isotype: IgG kappa

Information d'application

Indications d'application: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

SK-MEL-28 cells or Melanoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: gp100 / Melanosome / PMEL17 / SILV

Poids moléculaire: 90-100 kDa

ID gène: 6490, 95972

UniProt: P40967