Cyclin A anticorps

N° du produit ABIN6157632

Cet anticorps Souris Monoclonal détecte spécifiquement Cyclin A dans IF et FACS. Il présente une réactivité envers Humain, Souris, Boeuf (Vache), Green Monkey, Vison et Singe.

Aperçu rapide pour Cyclin A anticorps (ABIN6157632)

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivité: Humain, Souris, Boeuf (Vache), Green Monkey, Vison, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin A est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: E67

Détail du produit anti-Cyclin A anticorps

Fonction: Mouse Monoclonal anti-CyclinA2 (E67), Purified, BSA-free

Attributs du produit: This antibody recognizes a protein of 54 kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Full length bovine Cyclin A protein

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

K-562 and HeLa Cells

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur Cyclin A

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Autre désignation: CyclinA2

Sujet: CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2

Poids moléculaire: 54 kDa

ID gène: 890, 58974

UniProt: P20248

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA