TGFA anticorps (AA 34-43) (CF®647)

N° du produit ABIN6171418

L’anticorps Souris Monoclonal anti-TGFA a été validé pour IHC, IF et FACS. Il convient pour détecter TGFA dans des échantillons de Humain, Lapin et Poisson zèbre (Danio rerio).

Aperçu rapide pour TGFA anticorps (AA 34-43) (CF®647) (ABIN6171418)

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivité: Humain, Lapin, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TGFA est conjugé à/à la CF®647

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: P-T1

Détail du produit anti-TGFA anticorps (CF®647)

Épitope: AA 34-43

Fonction: Mouse Monoclonal anti-TGF alpha (P/T1), CF647 Conjugate

Attributs du produit: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF? ? (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: A 10-amino acid synthetic peptide (aa34-43, PPVAAAVVSH) from human TGF?.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-paraffin 2-4 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Jurkat or Ramos cells. Testicular Carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur TGFA

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF alpha

Sujet: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

Poids moléculaire: 6 kDa

ID gène: 7039, 170009

UniProt: P01135

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway