BRCA1 anticorps (AA 445-620) (CF®647)

N° du produit ABIN6175484

Détail du produit anti-BRCA1 anticorps (CF®647)

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Épitope: AA 445-620

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BRCA1 est conjugé à/à la CF®647

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Fonction: Mouse Monoclonal anti-BRCA1 (BRCA1/1398), CF647 Conjugate

Attributs du produit: The BRCA1 gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human BRCA1 protein fragment (aa445-620) (exact sequence is proprietary)

Clone: BRCA1-1398

Isotype: IgG1

Information d'application

Indications d'application: Immunohistology Formalin-fixed 5-10 μg/mL for 60 min at RT

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer  pH 6.0for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur BRCA1

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: BRCA1 (BRCA1 Produits)

Synonymes: anticorps BRCAI, anticorps BRCC1, anticorps BROVCA1, anticorps IRIS, anticorps PNCA4, anticorps PPP1R53, anticorps PSCP, anticorps RNF53, anticorps iris, anticorps pscp, anticorps brcai, anticorps brcc1, anticorps rnf53, anticorps brca1, anticorps BRCA1, anticorps LOC100224649, anticorps BRCA1, DNA repair associated, anticorps breast cancer 1, anticorps breast cancer 1, early onset, anticorps breast cancer 1 L homeolog, anticorps BRCA1, anticorps brca1, anticorps LOC755086, anticorps Brca1, anticorps brca1.L

Poids moléculaire: 220 kDa

ID gène: 672, 194143

UniProt: P38398

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus