GRP94 anticorps (CF®568)

N° du produit ABIN6179632

L’anticorps Rat Monoclonal anti-GRP94 a été validé pour WB, IF, FACS et IHC (f). Il convient pour détecter GRP94 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval et Xenopus laevis.

Aperçu rapide pour GRP94 anticorps (CF®568) (ABIN6179632)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval, Xenopus laevis

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP94 est conjugé à/à la CF®568

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 9G10-F8-2

Détail du produit anti-GRP94 anticorps (CF®568)

Fonction: Rat Monoclonal anti-Glucose Regulated Protein 94 (GRP94) (9G10.F8.2), CF568 Conjugate

Attributs du produit: This antibody recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Purified glucose regulated protein 94 (grp94) from chicken oviducts

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunohistology (Formalin-fixed) 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 0.5-1.0 μg/mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

U20S cells. About 50% of breast tumors express grp94 protein

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: Glucose Regulated Protein 94

Poids moléculaire: 94 kDa

ID gène: 7184, 192374

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades