Melanoma gp100 anticorps (CF®594)

N° du produit ABIN6190016

Cet anticorps Souris Monoclonal détecte spécifiquement Melanoma gp100 dans IHC, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour Melanoma gp100 anticorps (CF®594) (ABIN6190016)

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Melanoma gp100 est conjugé à/à la CF®594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HMB45

Détail du produit anti-Melanoma gp100 anticorps (CF®594)

Fonction: Mouse Monoclonal anti-Pmel17 / gp100 / SILV (HMB45), CF594 Conjugate

Attributs du produit: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Extract of pigmented melanoma metastases from lymph nodes

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Does not react with dog or rat, others not tested
• Optimal dilution for a specific application should be determined by user

Commentaires:

SK-MEL-28 cells or Melanoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: Pmel17 / gp100 / SILV

Poids moléculaire: 90-100 kDa

ID gène: 6490, 95972

UniProt: P40967