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Melanoma gp100 anticorps (CF®594)

Cet anticorps anti-Melanoma gp100 est un anticorps Souris Monoclonal détectant Melanoma gp100 dans IF, FACS et IHC (f). Adapté pour Humain et Cheval.
N° du produit ABIN6190054

Aperçu rapide pour Melanoma gp100 anticorps (CF®594) (ABIN6190054)

Antigène

Voir toutes Melanoma gp100 (PMEL) Anticorps
Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité

  • 112
  • 12
  • 8
  • 7
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Humain, Cheval

Hôte

  • 61
  • 49
  • 2
  • 1
Souris

Clonalité

  • 62
  • 49
  • 2
Monoclonal

Conjugué

  • 56
  • 9
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Melanoma gp100 est conjugé à/à la CF®594

Application

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  • 60
  • 42
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  • 26
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  • 7
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  • 1
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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

NKI-beteb
  • Fonction

    Mouse Monoclonal anti-Pmel17 / gp100 / SILV (NKI-beteb), CF594 Conjugate

    Attributs du produit

    By immunohistochemistry this antibody specifically recognizes a in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogène

    Membranes from a human melanoma metastasis

    Isotype

    IgG2b kappa
  • Indications d'application

    Immunohistology formalin-fixed 1-2 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Does not react with rat, others not tested
    • Optimal dilution for a specific application should be determined by user

    Commentaires

    SK-MEL-28 cells or Melanoma

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    100 μg/mL

    Buffer

    PBS/0.1 % BSA/0.05 % azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Protect from light
  • Antigène

    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

    Autre désignation

    Pmel17 / gp100 / SILV

    Poids moléculaire

    90-100 kDa

    ID gène

    6490, 95972

    UniProt

    P40967
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