ATF6 anticorps (N-Term)

N° du produit ABIN6251795

Cet anticorps anti-ATF6 est un anticorps Souris Monoclonal détectant ATF6 dans WB et IHC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour ATF6 anticorps (N-Term) (ABIN6251795)

Antigène: ATF6 (Activating Transcription Factor 6 (ATF6))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATF6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: AF6229

Détail du produit anti-ATF6 anticorps

Épitope: N-Term

Purification: Protein G purified monoclonal antibody

Immunogène: A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Western blot: 2-4 μg/mL (2)

IHC (Paraffin): 5 μg/mL (1)

ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. ATF6 is seen in western blotting between 75~90KD.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur ATF6

Antigène: ATF6 (Activating Transcription Factor 6 (ATF6))

Autre désignation: ATF6

Sujet: ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.

ID gène: 22926

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response