Cyclin A anticorps

N° du produit ABIN6251854

L’anticorps Souris Monoclonal anti-Cyclin A a été validé pour WB, IF et FACS. Il convient pour détecter Cyclin A dans des échantillons de Humain, Souris, Boeuf (Vache), Green Monkey et Vison.

Aperçu rapide pour Cyclin A anticorps (ABIN6251854)

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivité: Humain, Souris, Boeuf (Vache), Green Monkey, Vison

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: E67

Détail du produit anti-Cyclin A anticorps

Purification: Protein G affinity chromatography

Immunogène: Full length bovine protein was used as the immunogen for this CCNA2 antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL





The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Cyclin A

Antigène: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Autre désignation: Cyclin A2

Sujet: This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

ID gène: 890

UniProt: P20248

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA