CD47 anticorps

N° du produit ABIN6251897

Cet anticorps anti-CD47 est un anticorps Souris Monoclonal détectant CD47 dans FACS, WB, IHC, IF et BI. Adapté pour Humain.

Aperçu rapide pour CD47 anticorps (ABIN6251897)

Antigène: CD47

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD47 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), BioImaging (BI)

Clone: B6H12-2

Détail du produit anti-CD47 anticorps

Purification: Protein G affinity chromatography

Immunogène: Intact CD47 purified from placenta was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Functional Activity (Blocking): Order BSA/Azide-free format

Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Western blot: 2-5 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD47

Antigène: CD47

Autre désignation: CD47

Sujet: This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50  kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

ID gène: 961

UniProt: Q08722