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CD47 anticorps

CD47 Reactivité: Humain FACS, WB, IF, IHC, BI Hôte: Souris Monoclonal B6H12-2 unconjugated
N° du produit ABIN6251897
  • Antigène Voir toutes CD47 Anticorps
    CD47
    Reactivité
    • 119
    • 54
    • 28
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 80
    • 60
    • 10
    • 1
    Souris
    Clonalité
    • 75
    • 74
    • 1
    Monoclonal
    Conjugué
    • 65
    • 13
    • 12
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD47 est non-conjugé
    Application
    • 84
    • 83
    • 41
    • 41
    • 34
    • 26
    • 26
    • 18
    • 16
    • 13
    • 12
    • 10
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), BioImaging (BI)
    Purification
    Protein G affinity chromatography
    Immunogène
    Intact CD47 purified from placenta was used as the immunogen for this antibody.
    Clone
    B6H12-2
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CD47 Anticorps primaire
  • Indications d'application
    Functional Activity (Blocking): Order BSA/Azide-free format

    Flow Cytometry: 0.5-1 μg/million cells

    IF: 1-2 μg/mL

    IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

    Western blot: 2-5 μg/mL

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Antigène
    CD47
    Autre désignation
    CD47 (CD47 Produits)
    Synonymes
    anticorps IAP, anticorps MER6, anticorps OA3, anticorps 9130415E20Rik, anticorps AA407862, anticorps AI848868, anticorps AW108519, anticorps B430305P08Rik, anticorps Itgp, anticorps CD47/IAP, anticorps alkaline phosphatase, intestinal, anticorps CD47 molecule, anticorps CD47 antigen (Rh-related antigen, integrin-associated signal transducer), anticorps Cd47 molecule, anticorps ALPI, anticorps CD47, anticorps Cd47
    Sujet
    This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50  kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
    ID gène
    961
    UniProt
    Q08722
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