TOP2A
Reactivité: Humain
WB, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml
IF: 1-2 μg/mL
Western blot: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Antigène
Topoisomerase II alpha (TOP2A)
(Topoisomerase (DNA) II alpha 170kDa (TOP2A))
anticorps TOP2, anticorps TP2A, anticorps TOP2A, anticorps Top-2, anticorps TOP-2, anticorps F12B17.110, anticorps F12B17_110, anticorps thimet metalloendopeptidase 2, anticorps fc08c07, anticorps wu:fc08c07, anticorps DNA topoisomerase II alpha, anticorps DNA topoisomerase II, alpha isozyme, anticorps topoisomerase (DNA) II alpha, anticorps Zincin-like metalloproteases family protein, anticorps TOP2A, anticorps Bm1_08705, anticorps Top2a, anticorps AT5G10540, anticorps top2a
Sujet
This mAb recognizes an ~170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.