Neurofilament anticorps

N° du produit ABIN6252790

L’anticorps anti-Neurofilament Monoclonal Souris est utilisé pour la détection de Neurofilament dans des échantillons de Humain, Rat, Souris, Poulet et Porc. Il a été validé pour FACS, IHC et WB.

Aperçu rapide pour Neurofilament anticorps (ABIN6252790)

Antigène: Neurofilament

Reactivité: Humain, Rat, Souris, Poulet, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Neurofilament est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: NF421

Détail du produit anti-Neurofilament anticorps

Purification: Protein G purified Neurofilament antibody

Immunogène: Human recombinant Neurofilament protein was used as the immunogen. (1)

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (2)

Prediluted format : incubate for 30 min at RT (3)

The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Neurofilament

Antigène: Neurofilament

Sujet: Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68  kDa (NF-L), 160  kDa (NF-M) and 200  kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.

ID gène: 4744

UniProt: P12036