POFUT1 anticorps (C-Term)

N° du produit ABIN6257766

L’anticorps Lapin Polyclonal anti-POFUT1 a été validé pour WB, IHC, ELISA, IF et ICC. Il convient pour détecter POFUT1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour POFUT1 anticorps (C-Term) (ABIN6257766)

Antigène: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POFUT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-POFUT1 anticorps

Épitope: C-Term

Specificité: POFUT1 Antibody detects endogenous levels of total POFUT1.

Homologie: Pig,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human POFUT1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur POFUT1

Antigène: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Autre désignation: POFUT1

Sujet:

Description: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).

Gene: POFUT1

Poids moléculaire: 44 kDa

ID gène: 23509

UniProt: Q9H488

Pathways: Signalisation Notch, SARS-CoV-2 Protein Interactome