SIRT1 anticorps (AA 577-590)

N° du produit ABIN625834

Cet anticorps Chèvre Polyclonal détecte spécifiquement SIRT1 dans WB, IHC et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour SIRT1 anticorps (AA 577-590) (ABIN625834)

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SIRT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-SIRT1 anticorps

Épitope: AA 577-590

Fonction: Sirtuin 1 / SIRT1 (aa577-590)

Séquence: EKPQEVQTSR NVES

Specificité: This antibody is expected to recognize both reported isoforms (NP_036370.2, NP_001135970.1).

Réactivité croisée: Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2, NP_001135970.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Skin shows perinuclear staining in select keratinocytes. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 120 kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7 kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Ac

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur SIRT1

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Autre désignation: SIRT1

Sujet: SIRT1, sirtuin 1, SIR2L1, NAD-dependent deacetylase sirtuin-1, OTTHUMP00000198111, OTTHUMP00000198112, SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, sir2-like 1, sirtuin type 1

ID gène: 23411, 93759

NCBI Accession: NP_036370, NP_001135970

Pathways: Signalisation MAPK, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling