RAD51 Homolog B anticorps (Internal Region)

N° du produit ABIN6259179

L’anticorps anti-RAD51 Homolog B Polyclonal Lapin est utilisé pour la détection de RAD51 Homolog B dans des échantillons de Humain, Souris et Singe. Il a été validé pour WB, IHC, IF et ICC.

Aperçu rapide pour RAD51 Homolog B anticorps (Internal Region) (ABIN6259179)

Antigène: RAD51 Homolog B (Rad51B)

Reactivité: Humain, Souris, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD51 Homolog B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-RAD51 Homolog B anticorps

Épitope: Internal Region

Fonction: RAD51L1 Antibody

Specificité: RAD51L1 Antibody detects endogenous levels of total RAD51L1

Homologie: Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human RAD51L1, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur RAD51 Homolog B

Antigène: RAD51 Homolog B (Rad51B)

Autre désignation: RAD51L1

Sujet:

Description: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway.

Gene: RAD51B

Poids moléculaire: 45 kDa

ID gène: 5890

UniProt: O15315

Pathways: Réparation de l'ADN