ATG4A anticorps (N-Term)

N° du produit ABIN6260104

Cet anticorps anti-ATG4A est un anticorps Lapin Polyclonal détectant ATG4A dans WB, ELISA, IF et ICC. Adapté pour Humain et Souris.

Aperçu rapide pour ATG4A anticorps (N-Term) (ABIN6260104)

Antigène: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG4A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-ATG4A anticorps

Épitope: N-Term

Specificité: ATG4A Antibody detects endogenous levels of total ATG4A.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human ATG4A, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur ATG4A

Antigène: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Autre désignation: ATG4A

Sujet:

Description: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Gene: ATG4A

Poids moléculaire: 45 kDa

ID gène: 115201

UniProt: Q8WYN0

Pathways: Autophagy