MAP3K1 anticorps (AA 1211-1310)

N° du produit ABIN6294296

L’anticorps anti-MAP3K1 Monoclonal Souris est utilisé pour la détection de MAP3K1 dans des échantillons de Humain. Il a été validé pour WB, IHC, IF et FACS.

Aperçu rapide pour MAP3K1 anticorps (AA 1211-1310) (ABIN6294296)

Antigène: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAP3K1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-MAP3K1 anticorps

Épitope: AA 1211-1310

Fonction: Mouse anti-Human MAP3K1 Antibody [Sodium Azide Free]

Specificité: Cytoplasmic

Purification: A partial recombinant protein (aa 1211-1310) was used as the immunogen for the MAP3K1 antibody.

Immunogène: A partial recombinant protein (aa 1211-1310) was used as the immunogen for the MAP3K1 antibody.

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur MAP3K1

Antigène: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Autre désignation: Mitogen-activated protein kinase kinase kinase 1

Sujet:

Target Description: Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli, including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (MEK kinase- 5). MEK kinase-1 activates the ERK and c-Jun NH2-terminal kinase (JNK) pathways by phosphorylation of MAP2K1 and MAP2K4, and also activates the central protein kinases of the NF-kB pathway, CHUK and IKBKB. Additionally, MEK kinase-1 uses an E3 ligase through its PHD domain, a RING-finger-like structure, to target proteins for degradation through ubiquitination.

Gene Symbol: MAP3K1///MAPKKK1///MEKK///MEKK1

ID gène: 4214

UniProt: Q13233

Pathways: Signalisation MAPK, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades