PMEL anticorps (Premelanosome Protein)

Details for Product anti-PMEL Antibody No. ABIN6297733
Antigène
  • D12S53E
  • ME20
  • ME20-M
  • ME20M
  • P1
  • P100
  • PMEL17
  • SI
  • SIL
  • SILV
  • gp100
  • D10H12S53E
  • D12S53Eh
  • Pmel17
  • Si
  • Silv
  • gp87
  • RPE1
  • MMP115
  • silver
  • cb397
  • fdv
  • pmel17
  • sb:cb397
  • silva
  • wu:fc11g11
  • wu:fj24g11
  • zgc:136622
  • premelanosome protein
  • premelanosome protein a
  • PMEL
  • Pmel
  • pmela
Reactivité
Humain
214
32
10
7
6
4
1
1
1
1
Hôte
Souris
150
62
1
Clonalité
Monoclonal
Conjugué
Cet anticorp PMEL est non-conjugé
8
7
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
107
107
104
68
37
35
22
21
6
5
4
3
2
Options
Fonction Mouse anti-Human gp100 Antibody [Sodium Azide Free]
Immunogène The extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.
Specificité Cytoplasmic
Purification The extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.
Plasmids, Primers & others Plasmids, Primers & others PMEL products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Melanocyte protein PMEL , M-beta] (PMEL Antibody Extrait)
Sujet

Target Description: Melanocytes produce organelles called melanosomes which produce melanin, the pigment that gives color to skin, hair, eyes, scales and feathers. gp100 was identified in an attempt to clone the gene Tyrosinase, an enzyme required for melanin synthesis. Further testing determined that gp100 is a melanoma-specific protein and is responsible for melanosome maturation, facilitating the transition from amorphous rounded vesicles to fibrillary ellipsoid organelles.
Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Clone HMB45 gp100 antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. It also stains angiomyolipomas, tumors most commonly associated with the kidney. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. This gp100 antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Gene Symbol: PMEL///D12S53E///PMEL17///SILV

ID gène 6490
UniProt P40967
Indications d'application Western Blot: 1-2 μg/mL
FACS:0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Agent conservateur Azide free
Stock 4 °C,-20 °C
Stockage commentaire 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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