HNF4A anticorps (Internal Region)

N° du produit ABIN6391399

Cet anticorps Chèvre Polyclonal détecte spécifiquement HNF4A dans ELISA et IF. Il présente une réactivité envers Souris.

Aperçu rapide pour HNF4A anticorps (Internal Region) (ABIN6391399)

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp HNF4A est non-conjugé

Application: ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-HNF4A anticorps

Épitope: Internal Region

Fonction: HNF4A

Séquence: HPHLMQEHMG TN

Specificité: This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Preliminary experiments showed an approx 55 kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8 kDa according to NP_000448.3). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur HNF4A

Antigène: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Autre désignation: HNF4A

Sujet: HNF4A, hepatocyte nuclear factor 4, alpha , FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, HNF4-alpha, hepatic nuclear factor 4 alpha, hepatocyte nuclear factor 4 alpha, transcription factor-14

ID gène: 3172

NCBI Accession: NP_849180, NP_000448, NP_787110, NP_001025174, NP_001245284, NP_001274111, NP_001274112

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process