SMAD2 anticorps (pThr220)

N° du produit ABIN650816

L’anticorps anti-SMAD2 Polyclonal Lapin est utilisé pour la détection de SMAD2 dans des échantillons de Humain. Il a été validé pour WB, IF et DB.

Aperçu rapide pour SMAD2 anticorps (pThr220) (ABIN650816)

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Clone: RB18943

Détail du produit anti-SMAD2 anticorps

Épitope: pThr220

Homologie: B, Zf, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène: This SMAD2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T220 of human SMAD2.

Isotype: Ig Fraction

Information d'application

Indications d'application: IF: 1:10~50. WB: 1:500. DB: 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Détails sur SMAD2

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Autre désignation: SMAD2

Sujet: SMAD2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF?receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8), the common partner, (Smad4) that functions via its interaction to the various Smad's, and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.

Poids moléculaire: 52306

ID gène: 4087

NCBI Accession: NP_001003652, NP_005892

UniProt: Q15796

Pathways: Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus