CD25 anticorps (FITC)

N° du produit ABIN6655112

Détail du produit anti-CD25 anticorps (FITC)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD25 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Réactivité croisée (Details): Cross reactivity with CD25 from other sources has not been tested

Purification: Fluorescein conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25. Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Pigtailed Macaque. . Anti-CD25 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Immunogène:

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.

Clone: BC96

Isotype: IgG1

Labeling Ratio: 4-6

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CD25 is useful for Flow Cytometry and Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Références pour anticorps anti-CD25 (FITC) (ABIN6655112)

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." dans: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

Waldmann: "The interleukin-2 receptor." dans: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, (1991) (PubMed).

Détails sur CD25

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25 (IL2RA Produits)

Synonymes: anticorps CD25, anticorps IDDM10, anticorps IL2R, anticorps TCGFR, anticorps IL2RAC, anticorps IL-2RA, anticorps Il2r, anticorps Ly-43, anticorps interleukin 2 receptor subunit alpha, anticorps interleukin-2 receptor subunit alpha-like, anticorps interleukin 2 receptor, alpha chain, anticorps IL2RA, anticorps Il2ra, anticorps LOC100222957

Sujet:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55

Background: CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Gene Name: IL2RA

ID gène: 3559

NCBI Accession: NP_000408

UniProt: P01589

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation