MITF anticorps

N° du produit ABIN6655359

N° du produit (Fournisseur): 200-301-h93

Cet anticorps Souris Monoclonal détecte spécifiquement MITF dans WB, IHC et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour MITF anticorps (ABIN6655359)

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MITF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 21D1418

Détail du produit anti-MITF anticorps

N° du produit (Fournisseur): 200-301-h93

Fournisseur: Rockland

Fonction: MITF Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with MITF from Human, Rat, and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-MITF Antibody was purified by Protein G chromatography.

Immunogène: MITF Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide (sequence PSTGLCSPDLVN) corresponding to internal amino acids with human MITF protein.

Isotype: IgG1

Information d'application

Indications d'application: Optional[Neutralization_Dilution]: 3-5 μg/mL

Commentaires:

Anti-MITF antibody is tested for WB, IHC, and IF. Expect a band approximately 52kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur MITF

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Autre désignation: MITF

Sujet:

Synonyms: BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32

Background: Anti-MITF antibody detects human MITF. Microphthalmia-associated bHLH-LZ transcription factor (MITF) is a positively acting transcription factor containing a transcription activation domain and a bHLH-LZ domain that mediates DNA-binding and dimerization. Both the transcription activation and bHLH-LZ domains are highly conserved in the closely related TFE3, TFEB and TFEC. MITF can form heterodimers in vitro with the above transcription factors, but not with other bHLH-LZ factors such as Myc, Max or USF. MITF is expressed as multiple isoforms, which share common activation and DNA-binding domains, but differ in the amino-terminal region. MITF is targeted by three kinases: GSK-3beta, Map kinase ERK2 and members of the p90 Rsk family of kinases. Anti-MITF antibody is ideal for investigators involved in kinase, phosphatase, NFkappaB, and signaling research.

Gene Name: MITF

ID gène: 4286

NCBI Accession: NP_000239

UniProt: O75030

Pathways: Chromatin Binding