Beclin 1 anticorps (pSer234)

N° du produit ABIN6655419

N° du produit (Fournisseur): 600-401-w96

Cet anticorps Lapin Polyclonal détecte spécifiquement Beclin 1 dans WB et ELISA. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour Beclin 1 anticorps (pSer234) (ABIN6655419)

Antigène: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Beclin 1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-Beclin 1 anticorps

Épitope: pSer234

N° du produit (Fournisseur): 600-401-w96

Fournisseur: Rockland

Fonction: BECLIN1 phospho S234 Antibody

Réactivité croisée (Details): Anti-Beclin1 pS234 Antibody is directed against Beclin 1 protein phosphorylated at S234.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Anti-Phospho-Ser234 Beclin-1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of human Beclin-1 conjugated to KLH.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:2,500

Western_Blot_Dilution: 1:250

Commentaires:

Suggested Applications: ELISA
Anti-Phospho-Ser234 Beclin-1 Antibody is tested for WB. Expect a band at approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user. Immunolabeling of the Beclin-1 band is decreased in the Beclin-1 alanine substitution mutant.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Beclin 1 (ABIN6655419)

Xie, Kang, Tang: "Assessment of Posttranslational Modifications of ATG proteins." dans: Methods in enzymology, Vol. 587, pp. 171-188, (2017) (PubMed).

Détails sur Beclin 1

Antigène: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Autre désignation: Beclin1

Sujet:

Synonyms: Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein

Background: Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies. Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234. Beclin 1 pS234 Antibody is ideal for researchers interested in cancer research.

Gene Name: BECN1

ID gène: 8678

NCBI Accession: NP_001300927

UniProt: Q14457

Pathways: Autophagy