Caspase 3 anticorps

N° du produit ABIN6655470

N° du produit (Fournisseur): 200-301-h63

Cet anticorps Souris Monoclonal détecte spécifiquement Caspase 3 dans WB, IHC, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour Caspase 3 anticorps (ABIN6655470)

Antigène: Caspase 3 (CASP3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caspase 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 31A1067

Détail du produit anti-Caspase 3 anticorps

N° du produit (Fournisseur): 200-301-h63

Fournisseur: Rockland

Fonction: Caspase-3 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-3 from Human, mouse and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Caspase-3 Antibody was purified by Protein G chromatography.

Immunogène: Caspase-3 Antibody was produced in mice by repeated immunizations with a full length recombinant Caspase-3 human protein.

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 5 μg/mL

Western_Blot_Dilution: 1-5 μg/mL

Commentaires:

Anti-Caspase 3 Antibody is tested for use in WB, EM, Flow, ICC/IF, IHC-Fr, and IHC-P. Expect a band approximately 31.5 kDa on specific lysates. The antibody detects both pro Caspase-3(32 kDa) and the large subunit of the active/cleaved form (14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Caspase 3 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Caspase 3

Antigène: Caspase 3 (CASP3)

Autre désignation: Caspase-3

Sujet:

Synonyms: Cpp32, Caspase-3 subunit p17, Caspase-3 subunit p12, Apopain, Cysteine protease CPP32 Caspase-3, CASP-3, 3.4.22.56, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12

Background: Anti-Caspase-3 antibody detects human Caspase-3. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32- kDa protein. Caspase-3 cleaves the death substrate poly (ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17- kDa and a small subunit of 12- kDa. Anti-Caapase-3 is ideal for investigators involved in apoptosis, cytokines and growth factor research.

Gene Name: Casp3

ID gène: 836

NCBI Accession: NP_004337

UniProt: P42574

Pathways: Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation