Tumor Protein p73 anticorps

N° du produit ABIN6655655

N° du produit (Fournisseur): 200-301-i00

Cet anticorps anti-Tumor Protein p73 est un anticorps Souris Monoclonal détectant Tumor Protein p73 dans WB, IHC, IF, IP, FACS et ChIP. Adapté pour Humain.

Aperçu rapide pour Tumor Protein p73 anticorps (ABIN6655655)

Antigène: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Tumor Protein p73 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP)

Clone: 5B429

Détail du produit anti-Tumor Protein p73 anticorps

N° du produit (Fournisseur): 200-301-i00

Fournisseur: Rockland

Fonction: p73 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-p73 from Human and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-p73 Antibody was purified by Protein G chromatography.

Immunogène: p73 Antibody was produced in mice prepared by repeated immunizations with full-length human p73 protein.

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: 5-15 μg/mL

Western_Blot_Dilution: 2 μg/mL

Commentaires:

Anti-p73 Antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 70kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Tumor Protein p73

Antigène: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Autre désignation: p73

Sujet:

Synonyms: P73, Tumor protein p73, p53-like transcription factor, p53-related protein

Background: Anti-p73 detects human p73. NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBa. Anti-p73 antibody is ideal for investigators involved in NFkappaB, kinase and phosphatse research.

Gene Name: TP73

ID gène: 7161

NCBI Accession: NP_001119712

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus