SOD2 anticorps

N° du produit ABIN6656073

N° du produit (Fournisseur): 600-401-g14

Cet anticorps Lapin Polyclonal détecte spécifiquement SOD2 dans WB, IHC, ELISA et IP. Il présente une réactivité envers Humain.

Aperçu rapide pour SOD2 anticorps (ABIN6656073)

Antigène: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SOD2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Détail du produit anti-SOD2 anticorps

N° du produit (Fournisseur): 600-401-g14

Fournisseur: Rockland

Fonction: SOD2 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with SOD2 from Human, Rat, Mouse, Bovine, Canine, Chicken, Gerbil, Guinea Pig, Pig, Hamster, Monkey, Rabbit, Sheep, and Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-SOD2 Antibody was purified by affinity chromatography.

Stérilité: Sterile filtered

Immunogène: SOD2 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against human SOD2.

Isotype: IgG

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 0.2 μg/mL

Commentaires:

Anti-SOD2 Antibody is tested for use in WB, IP, and IHC. Expect a band approximately ~25kDa protein corresponding to the molecular mass of SOD2 in WB. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur SOD2

Antigène: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Autre désignation: Mn SOD

Sujet:

Synonyms: Manganese SOD, IPO B, Mn SOD, SOD2, Superoxide dismutase [Mn], mitochondrial

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. In general, SODs play a major role in antioxidant defense mechanisms. There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity.

Gene Name: SOD2

ID gène: 6648

NCBI Accession: NP_000627

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling